WebIn contrast, in the spheroids treated with cisplatin (33 μM) an evident increase in the activity of these caspases is observed up to 1500 RLUs, indicating induction of apoptosis. To confirm the lack of apoptosis activation, the activity of initiator caspases 8 and 9 was measured through the effect of specific inhibitors on cell viability of ... WebMar 17, 2016 · Induction of ROS-dependent apoptosis by cisplatin combined with gallic acid (GA) in H446 cells. (A) Apoptosis assay by flow cytometry using PI and Annexin V-FITC double staining. H446 cells were treated with or without 6 mM NAC for 2 h before challenge with 5 µg/ml cisplatin combined with 3 µg/ml GA for 24 h.
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WebMay 16, 2011 · After these metabolic changes a drastic decrease in cellular impedance occurred at about 10–11 h after exposure to cisplatin ( Fig. 2 C ), which reflects the … WebCisplatin activates multiple signal transduction pathways involved in coordinating cellular responses to stress. Here we demonstrate a requirement for extracellular signal-regulated protein kinase (ERK), a … grand design ultra light fifth wheels
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WebAug 16, 2002 · Induction of apoptosis with MMC in the human breast cancer cell line MCF-7. To determine the cytotoxic effect of various anticancer drugs with different mechanisms of action in MCF-7 cells, the ... WebThe anticancer drug cisplatin induces cell death by apoptosis. Apoptosis is dependent on cellular loss of potassium ions (K+). We have recently shown that the antifungal drug amphotericin B (enhancin WebJul 5, 2024 · The obtained results from flow cytometry analysis showed that the rate of apoptosis was 4.26% in the cells treated with cisplatin, 38.69% in the cells treated with cisplatin-IONPs and 46.33% in the cells treated with cisplatin-IONPs-chitosan after 24 h (Fig. 3) and also showed that the rate of necrotic cells treated with cisplatin was … grand design travel trailers review