Hct116 puromycin
WebMar 11, 2016 · Most recent answer 11th Dec, 2024 Ching Yi Ong Universiti Sains Malaysia Hi, I am working on HCT116 and HT29 cells. May I know what was the concentration of … WebGibco® fetal bovine serum, puromycin, ... HCT116 and HT29 cells were cultured in growth medium (DMEM supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin) in a humidified atmosphere with 5% CO2 at 37 °C. Both malignant cell lines were stably transfected with shSep15 or a control construct
Hct116 puromycin
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WebPuromycin is an aminonucleoside antibiotic produced by S. alboniger. 15 It blocks protein synthesis by interacting with the A site of the large ribosomal subunit of both prokaryotic and eukaryotic ribosomes, 16 because it resembles an amino acid attached to the terminal adenosine of tRNA. This interaction is followed by peptide bond formation ... WebMar 14, 2024 · Puromycin was bought from Sigma-Aldrich (St. Louis, MO, USA). Firefly luciferase-lentiviruses and Polybrene were obtained from GENECHEM (Shanghai, China). D-luciferin potassium salt was obtained from PROMEGA (Madison, WI, USA). ... Compared to HCT116 and SW620 tumor-bearing mice, other groups (SW480, HT29, LOVO and …
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WebApr 24, 2024 · Notably, puromycin upregulated the expression of RPL5 and RPL11 to directly bind to MDM2 in HCT116 cells. Conversely, deletion of RPL5 and RPL11 … WebApr 1, 2024 · After 72 h of transfection, 2 mg/ml puromycin was added to the culture medium for HCT116 or RKO selection, respectively. ... A HCT116 cells in the siHOXC6 group were more sensitive to 5-Fu, ...
WebHCT116 knockout cells were infected with a blank or a p53-expressing lentivirus; HCT116 parental p53 wt cells were infected with a blank …
Web7 rows · Puromycin; 0.8μg/ml; 322: HCT 116: phMLH1; pcDNA3.1/Hygro; Hygromycin; 100μg/ml; 525: HCT 116: pcDNA3.1(+) Geneticin (G418) 600μg/ml; 546: HCT 116: … push notifications chrome macbookWebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram-positive bacteria and various animal and insect cells. sedgwick des moines officeWebApr 7, 2024 · In line with these, we observed protein levels of DDX21 in most of CRC cell lines, such as HCT116, LOVO and RKO, are significantly higher than those in normal colonic epithelial cell NCM460 except ... sedgwick des moines iowaWebGeneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishment. Explore all Gibco selection antibiotics sedgwick detert moran arnoldWebAug 29, 2002 · A colorectal carcinoma cell line, HCT116, which has both mutated and wild-type β-catenin genes, was engineered by homologous recombination to investigate the significance of β-catenin gene mutation. sedgwick detert moran and arnoldWebHCT 116 Cell Line. HCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into … sedgwick dhlhttp://cell-lines.toku-e.com/Cell-Lines_1422.html sedgwick des moines ia